CmSERK gene is expressed in response to the attack of pathogen fungi Colletotrichum acutatum and Botrytis cinerea
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Jun 24, 2021
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Abstract
The genus Cattleya groups orchids originate in tropical zones of South and Central America. One of the most representative species of ornamental importance is Cattleya maxima Lindl. In this study the fungal pathogens Colletotrichum acutatum and Botrytis cinerea were isolated and their pathogenicity was determined by in vitro inoculation of Cattleya maxima. Pathogenicity tests resulted positive for infection with C. acutatum after seven days of inoculation while as for B. cinerea the symptoms of infection appeared after two days. Quantitative PCR revealed that CmSERK gene is more expressed in tissue under fungal attack. These results suggest that CmSERK gene plays an important role in the activation of defense-related responses.
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RiveraP., RojasJ., ConciaL., & Cueva AgilaA. Y. (2021). CmSERK gene is expressed in response to the attack of pathogen fungi Colletotrichum acutatum and Botrytis cinerea. AXIOMA, 1(24), 64-69. https://doi.org/10.26621/ra.v1i24.688
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CIENCIAS NATURALES, MATEMÁTICAS Y ESTADÍSTICA
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Wu S, Shan L, He P. 2014. Microbial signature-triggered plant defense responses and early signaling mechanisms. Plant Sci. 228C:118–126. https://doi.org/10.1016/j.plantsci.2014.03.001.
Barnett, H y Hunter, B. 1998. Illustrated Genera of Imperfect Fungi. Cuarta edicion. The American Psychopathological Society. Pp. 188-189.
Becraft P.W. 1998. Receptor kinases in plant development. Trends Plant. 1998; Sci 3:384–388
Bigeard, J.1., Colcombet, J.1, Hirt H. 2015. Signaling mechanisms in pattern-triggered immunity. Mol Plant. 8(4):521-39. https://doi.org/10.1016/j.molp.2014.12.022
Cannon, P.F., Damm, U., Johnston, P.R., Weir, B. S. 2012. Colletotrichum current status and future directions. Studies in Mycology 73, 181-213. https://doi.org/10.3114/sim0014.
Cueva, A., Concia, L., Cella, R. 2012. Molecular characterization of a Cyrtochilum loxense Somatic Embryogenesis Receptor-like Kinase (SERK) gene expressed during somatic embryogenesis. Plant Cell Rep, Volume 31, Issue 6. Pp. 1129 – 1139.
Cueva-Agila, A.Y., Alberca-Jaramillo, N., Cella, R. and Concia, L. 2020. Isolation, phylogenetic analysis, and expression of a Somatic Embryogenesis Receptor like Kinase (SERK) gene in Cattleya maxima Lindl. Current Plant Biology. https://doi.org/10.1016/j.cpb.2020.100139.
Dodson, C., Luerand, C. y Gustavo L. 2004. Orchids of Ecuador. Soluciones Gráficas D Yg. Quito – Ecuador.
Hecht, V., Velle-Calzada, J.P., Hartog, M.V., Schmidt, E.DL, Boutilier K, Grossniklaus U, de Vries SC. 2001. The Arabidopsis somatic embryogenesis receptor kinase 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol. 127: 803–816. https://doi.org/10.1104/pp.010324
Hou, C. y Yang, C. 2009. Functional Analysis of FT and TFL1 Orthologs from Orchid (Oncidium Gower Ramsey) that Regulate the Vegetative to Reproductive Transition. Plant and Cell Physiology, Vol. 50, No. 8., pp. 1544-1557. https://doi.org/10.1093/pcp/pcp099Hu, H., Xiong, L., Yang, Y. 2005. Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection; Planta 222:107–117. https://doi.org/10.1007/s00425-005-1534-4
Latorre, B.A.; Agostín, E.; San Martín, R.; Vázquez, G.S. 1997. Effectiveness of conidia of Trichoderma harzianum produced by liquid fermentation against Botrytis bunch rot of table grape in Chile. Crop Protection.; 16: 209- 214.
Pfaffl, M.W. 2001 A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9): e45.
Prihastuti, H., Cai, L., Chen, H., Mckenzie, E.H.C. and Hyde K.D. 2009. Characterization of Colletotrichum species associated with coffe berries in northen Thailan. Fungal Diversity. 89-109
Santos, M.O., Romano, E., Vieira, L.S, Baldoni,A.B, Aragão, F.J.L. 2008. Suppression of SERK gene expression affects fungus tolerance and somatic embryogenesis in transgenic lettuce. Plant Biol. doi:10.1111/j.1438-8677.2008.00103.x.
Schmidt, E.D., Guzzo, F., Toonen, M.A., De Vries, S.C. 1997. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development. ; 124:2049–2062
Sessa, G. and Martin, G. 2000. Protein Kinases in the Plant Defense Response. Advances in Botanical Research, 32:379-404. https://doi.org/10.1016/S0065-2296(00)32030-4.
Tomas-Grau, R., Di Pieto, P.,., Chalfoun, N.., Grellet-Bournonville, C., Martos GG., debes M., Arias M E., Diaz-Ricci J., 2019. Colletotrichum acutatum M11 can suppress the defence response in strawberry plants. Planta DOI: https ://doi.org/10.1007/s0042 5-019-03203 -5.
Walker, A.-S., Gautier, A., Confais, J., Martinho, D., Viaud, M., Le Pêcheur, P., Dupont, J., and Fournier, E. 2011. Botrytis pseudocinerea, a new cryptic species causing gray mold in French vineyards in sympatry with Botrytis cinerea. ; Phytopathology 101:1433-1445. https://doi.org/10.1094/PHYTO-04-11-0104
Wan WL, Fröhlich K, Pruitt RN, Nürnberger T, Zhang L 2019. Plant cell surface immune receptor complex signaling. Curr Opin Plant Biol.15;50:18-28. doi: 10.1016/j.pbi.2019.02.001. https://doi.org/10.1016/j.pbi.2019.02.001.
White, T.J., Bruns, T., Lee, S., and Taylor, J. 1990. “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics,” in PCR Protocols: a Guide to Methods and Applications, eds. M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White. San Diego, USA: Academic Press, https://doi.org/10.1007/s10658-016-1009-4,
Wit, P. 2007. How plants recognize pathogens and defend themselves. Cell Mol Life Sci. ;64: 2726 – 2732. https://doi.org/10.1007/s00018-007-7284-7.
Wu S, Shan L, He P. 2014. Microbial signature-triggered plant defense responses and early signaling mechanisms. Plant Sci. 228C:118–126. https://doi.org/10.1016/j.plantsci.2014.03.001.