Apitoxin, an attenuator of the "intelligence" of pathogenic enterobacteria for guinea pigs (cavia porcellus)
Article Sidebar
Main Article Content
Abstract
The presence of enterobacterial diseases in guinea pigs (Cavia porcellus), caused by Escherichia coli, Yersinia pseudotuberculosis and Salmonella typhimorium, as well as the excessive use of antibiotics and other medicines that leave residual actions in animal meat, constitute the basic elements of the problem which originated this research that was developed in three phases: a) determination of the minimum inhibitory concentration (MIC) of apitoxin for enterobacteria of guinea pigs in the fattening phase, b) action of apitoxin in the treatment of clinically diseased guinea pigs by enterobacteria, and c ) solutions of apitoxin as a preventive for the enterobacteria attack in guinea pigs. Therefore, the objective of this third phase, the reason for this article, was to determine the effects of simultaneous dilutions of 0.7 and 0.8 mg / l-10 of apitoxin in enterobacteria cultures, to attenuate their virulence or pathogenic intelligence, highlighting that these concentrations are the result of phases a and b of the research, related to the use of apitoxin in solutions against pathogenic enterobacteria for guinea pigs. After the trial development in 35 in 3-month old guinea pigs, the results confirmed the in vitro values of the MICs of 0.7 and 0.8 mg / l-10 with 10 simultaneous dilutions for immunological protections for the three enterobacteria, the responses evaluated by thymus lymphoid organometry, spleen and mesenteric lymph in size (mm) and weight (g), as well as for guinea pig mortality. Concluding that the bee venom (Apis mellifera) has the possibility of attenuating the virulence of the enterobacteria under study.
Downloads
Article Details
Con la finalidad de contar con un tipo de licencia más abierta en el espectro que ofrece Creative Commons, a partir de diciembre de 2022 desde el número 27, AXIOMA asume la Licencia Creative Commons 4.0 de Reconocimiento-NoComercial-CompartirIgual 4.0(CC BY-NC-SA 4.0). Tanto el sitio web como los artículos en sus diferentes formatos, reflejan esta información.
Hasta el mes de noviembre de 2022 con el número 26, la revista AXIOMA asumió una Licencia Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0). Los artículos contenidos en cada número hasta el 26, cuentan con esta licencia y su descripción se conserva en el portal de nuestra revista.
Atribución-NoComercial-SinDerivadas
CC BY-NC-ND
AXIOMA- Revista Científica de Investigación, Docencia y Proyección Social
References
Akbar K.; Farhad, A.; Kazem, P.; Mohammad, N.; Saied, H.; Soharab, I. Hossein, A. (2012). Effect of Honey Bee Venom on Lewis Rats with Experimental Allergic Encephalomyelitis, a Model for Multiple Sclerosis, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran J Pharm Res. Spring;11(2):671-8.
Arteaga, V.; Jáuregui, D. (2017). Incidencia de la apitoxina en los órganos linfáticos en pollos broiler. En Avances, desarrollo y sustentabilidad agroambiental en Ecuador y Venezuela, pp. 190-197. Venezuela: Universidad de Los Andes, Pontificia Universidad Católica del Ecuador.
Baek, Y. H., Huh, J. E.; Lee, J. D.; Choi, Do. Y, Park, D. S. (2006). Antinociceptive effect and the mechanism of bee venom acupuncture (Apipuncture) on inflammatory pain in the rat model of collagen-induced arthritis: Mediation by (2- adrenoceptors. Brain Res., 16, 305-310
Bartholomew, M.L., Heffernan, R.T., Wright, J.G., Klos, R.F., Monson, T., Khan, S., Trees, E., Sabol, A., Willems, R.A., Flynn, R., Deasy, M.P., Jones, B., Davis, J.P. (2014). Multistate Outbreak of Salmonella enterica Serotype Enteritidis Infection Associa-ted with Pet Guinea Pigs. Vector Borne Zoonotic Dis. 2014 Jun;14(6):414-21. doi: 10.1089/vbz.2013.1506
Caycedo, A., Zamora-Burbano, A., Echevarry-Potosí, S., Enríquez, R., Ortega-David, E., Burgos, M. Caycedo, M. (2011). Producción sostenible de cuyes. Colombia: Asindetec.
Cárdenas-Perea, M.E.; Cruz L., López, O.R.; Gándara-Ramírez, J.L.; Pérez-Hernández, M.A. (2014). Factores de Virulencia Bacteriana: La inteligencia de las Bacterias. Elementos, Revista de Ciencia y Cultura, 21(94). 35. Recuperado de: https://elementos.buap.mx/num94/htm
Castro, H.J.; Mendez-Lnocencio, J.I.; Omidvar, B.; Omidvar, J.; Santilli, J.; Nielsen, H.S.; Pavot, A.P.; Richert, J.R.; Bellanti, J.A. (2005), A Phase I Study of the safety of honey bee venom extract as a possible treatment for patients with progressive forms of multiple sclerosis. Allergy Asthma Pro., 26, 470-476. Recuperado de: https://www.ncbi.nlm.nih.gov/pubmed/16541972
Chauca, L. (2005). Producción de cuyes (Cavia porcellus). Coordinación de Crianzas Familiares. Instituto Nacional de Investigación Agropecuaria (INIA). La Molina- Perú.
Espíndola, J. L.; Espíndola, M. A. (2005). “La inteligencia”. En Editorial Pearson Educación, Pensamiento crítico, México: 5-9.
Fortoul Van Der Goes, T. (2017). Histología y biología celular, 3e. McGraw- Hill, Interamericana Editores, S. A. Ciudad de México.
Guerra, C. (2009). Potenciando capacidades para el desarrollo sostenible de Chetilla y Magdalena-Cajamarca. Manual Técnico de Crianza de Cuyes, 16 Perú.
Gutiérrez, P. J. (2010). Inmunología Veterinaria. Editorial Mundo Moderno, S. A. de C.V. México
Han, S.; Lee, K.; Yeo, J.; Kweon, H.; Woo, S.; Lee, M.; Baek, H.; Kim, S.; Park, K. (2007), Effect of honey Bee venom on microglial cells nitric oxide and tumor necrosis factor - α production stimulated by LPS. J. Enthnopharmacol. 111: 176-181.
Han, S. M.; Lee, K.G.; Yeo, J.H.; Oh, B.Y.; Kim, B.S.; Lee, W.; Baek, H.J.; Kim, S.T.; Hwang, S.J.; Pak, S.C. (2010). Efect of honeybee venom supplementation in drinking wáter on growth perfomance of broiler chickens. Poultry Science Association Inc, Korea, 42: 253-268, doi: 10.3382/pp. 2010-00915.
Han, S.M.; Lee, G.G.; Park, K.K. (2012). Skin Sensitization Study of Bee Venom (Apis mellifera L.) in Guinea Pigs. Toxico, 28:1-4.
Instituto Nacional Autónomo de Investigaciones Agropecuarias (INIAP) (2002). Producción de cuyes (Cavia porcellus). [en línea], disponible en: http://iniap.gob.ec
Jang, M.H. (2003), Bee venom induces apoptosis and inhibits expression of eyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299. J. Pharmacol. Sci., 91, 95-104.
Mahler, M., Berard, M., Feinstein, R., Gallagher, A., Illgen-Wilcke, B., Pritchett-Corning, K.; Raspa, M. (2014). FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. Laboratory Animals.48(3) 178–192.Doi: 10.1177/0023677213516312.
Matysiaka, J.; Christian, E.H.; Schmelzer, B.; Reinhard, H.H.; Neubert, B.; Zenon, J. K. (2011), Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry, Journal of Pharmaceutical and Biomedical Analysis 54 (2011) 273–278.
Park, M.H.; Choi, M.S.; Kwak, D.H.; Oh, K.W.; Yoon, D.Y.; Han, S.B.; Song, H.S.; Song, M.J.; Hong, J.T. (2010). Anti-cancer effect of bee venom in prostate cancer cells through activation of caspase pathway via inactivation of NF-B. Prostate, 17, 801-812
Ramírez, I. (2003). Estudio bacteriológico y epidemiológico de un brote infeccioso en cobayos (Cavia porcellus). (Tesis pregrado. Universidad. Nacional Mayor de San Marcos).
Randall, D.; Warren, B.; Eckert, R.; French, K. (2002). Fisiología Animal: mecanismos y adaptaciones. Universidad de Stanford. Editorial McGraw Hill, Buenos Aires-Argentina.
Son, D.J.; Lee, J.W; Lee, Y.H.; Song, H.S.; Lee, C.K.; Hong, J.T. (2007). Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds, Pharmacology & Therapeutics. 115 (2007) 246–270.
Torres, S. y Tirira, M. (2017). Incidencia de enterobacterias en cuyes de las parroquias de Natabuela y Chaltura. (Tesis de pregrado Pontificia Universidad Católica del Ecuador Sede Ibarra). Recuperado de://dspace.pucesi.edu.ec/handle/11010/6/browse?
Wagner, J. E.; Manning, P. J. (2014). The Biology of the Guinea Pig. Academic Press, INC (London), NewYork, LTD. ISBN: 0-12-730050-3
Wang, C.; Chen, T.; Zhang, N.; Yang, M.; Li, B.; Lü, X.; Cao, X.; Ling, C. (2009). Melittin, a major component of bee venom, sensitizes human hepatocellular carcinoma cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) - induced apoptosis by activating CaMKII-TAK1-JNK/p38 and inhibiting Ikappa Balpha kinase-NFkappaB. J. Biol. Chem., 284, 3804-3813.
Weigel, L.M.; Clewell, D.B.; Gill, S.R.; Clark, N.C.; McDougal, L.K.; Flannagan, S.E.; Kolonay, J.F.; Shetty, J.; Killgore, G.E.; Tenover, F.C. (2003). Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science 302(5650), 1569-1571.