Desarrollo y validación de un método en cromatografía líquida de alta eficiencia para la determinación depinocembrin en hojas de la especie piper ecuadorense sodiro

Abstract

Pinocembrin is a flavanon e that shows pharmacological and biological activities such as antifungal, anticancer, antimicrobial, anti-inflammatory, and antioxidants. This secondary metabolite has been identified in several plants belonging to the Piperaceae family. Piper ecuadorense is a native shrub used in traditional medicine by healers from the Saraguro indigenous community of Ecuador. Therefore, due his important activity, it is necessary develop and validate a method by using HPLC-DAD in order to determinate the pinocembrin in Piper ecuadorense leaf extracts. The method utilized a BDS Hipersil C-18 column (5 μm, 4.6x250 mm i.d.) with the mixture of ammonium acetate (0.01 M) and methanol (35:65 %, v/v) as the mobile phase at a flow-rate of 1 mL/min, and detection was by UV at 290 nm. The parameters of linearity, accuracy, precision, recovery, detection limit, and quantitation limit of the HPLC method were achieved. The method showed excellent linearity (r2 = 0.9998), and the recovery was 100.70-101.33%. A high degree of accuracy and precision (RSD less than 3% for intraday and interday) were likewise reached. In this way, a simple, precise, accurate and reproducible HPLC method was successfully developed and validated to analyze pinocembrin in Piper ecuadorense leaf extracts.