Estudio químico semicuantitativo y comparativo de la fracción volátil destilada desde la especie ecuatoriana Piper subscutatum (Miq.) C. DC. (Piperaciae

Abstract

This research work presents the comparison of two chemical analyzes carried out separately on two dates of the same year, to the volatile fractions distilled from leaves of the Piper subscutatum species, collected in Numbani, Zamora Chinchipe, Ecua-dor. The first of them was quantitative, distilling a volatile fraction by vapor drag, and analyzed with a flame ionization detector (FID). This analysis was previously published as "Essential Oil and Major Non-Volatile Secondary Metabolites from the Leaves of Amazonian Piper Subscutatum". We are now introducing the second analysis, which was semi-quantitative, made by mi-cro-hydrodistillation and analyzed with an mass spectrometry detector (MSD), and a gas chromatographer with polar and apolar columns. We found 45 common compounds between both studies, most of them hydrocarbon sesquiterpenes (55.65% and 41.68%), oxygenated sesquiterpenes (8.55% and 8.75%), hydrocarbon monoterpenes (8.25% and 28.72%). Also, there were, in lesser proportions, monoterpenes oxygenated (1.25% and 3.96%) and others (2.5% and 3.58%). Standard deviation was also compared. In both studies, the most abundant compound was the sesquiterpene E-caryophyllene. For the first study, the second and third places there were β-chamigreno and E-nerolidol, which have pharmacological uses. In the second study, therewere monoterpenes: α-pinene, and β-pinene in second and third place, displacing β-chamigreno and E-nerolidol from secondto ninth place and from third to fourth place respectively. We also made a review of the terpenes' activities to think about their pharmacological and agrochemical applications.